Label-Free Protein Analysis Using Liquid Chromatography with Gravimetric Detection

The detection and analysis of proteins in a label-free manner under native solution conditions is an increasingly important objective analytical bioscience platform development. Common approaches to detect often require specific labels enhance sensitivity. Dry mass sensing approaches, by contrast, using mechanical resonators, can operate offer attractive However, such typically suffer from lack analyte selectivity as the interface between standard protein separation techniques micro-resonator platforms constrained qualitative sensor performance liquid phase. Here, we describe strategy that overcomes this limitation coupling chromatography with quartz crystal microbalance (QCM) microfluidic spray dryer. We explore which allows first separate mixture physiological buffer size exclusion chromatography, permitting fractions be selected, desalted, subsequently spray-dried onto QCM for absolute analysis. By establishing continuous flow column device via splitter, simultaneous sample fractionation achieved, sensitivity down 100 ?g/mL limit detection. This approach quantitative offers potential species conditions.